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Objective:To establish a method for determintation of chlorogenic acid and linarin in Yejuhua granules by HPLC.Methods:We applied HPLC methods. The Kromasil 100-5 C18 column (250 mm×4.6 mm,5 μm) was used, the mobile phase was acetonitrile-0.4%H 3PO 4 solution (gradient elution), the flow rate was 1.0 ml/min, the dection wavelenghth was 334 nm and the column temperture was 32 ℃. Results:Chlorogenic acid and buddleoside had good linearity in the ranges of 0.30-1.50 μg ( r2=0.999 1) and 0.12-0.62 μg ( r2=0.999 8), respectively. The average recoveries were 99.70% and 96.67%, with RSD<2%, respectively. Conclusion:The method is simple, rapid, reliable, efficient, and can be used for determination of chlorogenic acid and buddleoside in Yejuhua Granules.
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Objective To investigate the relationship between BMI and blood lipids, blood glucose and blood uric acid. Methods A total of 3 860 employees from a university who underwent physical examination in 2018 were selected and divided into obesity group, fat group, normal group and lean group according to their body mass index. The levels of blood lipids, fasting blood glucose and blood uric acid among different groups were analyzed and compared. Results There were a total of 1,889 people in the obesity group and fat group, accounting for 48.94% of the total. The weight difference among the different groups was statistically significant (P＜0.05), while there was no statistical significance in age difference among different groups. The height in the obese group and fat group was significantly different compared with the normal group. There were significant differences in the levels of triglyceride (TG), high-density lipoprotein (HDL-C) and blood uric acid (UA) in the different groups (P<0.05). The fasting blood glucose level (GLU) in both the obesity group and fat group was significantly different compared with the normal group (P<0.05). With the increase in body weight index, the proportion of high blood fat, fatty liver, hyperglycemia and high blood uric acid all increased significantly as compared with the normal group or the lean group, and the differences were statistically significant (P<0.05). The proportion of fatty liver and hyperuricemia in the male was higher than that in the female. Conclusion The risk of high blood fat, fatty liver, hyperglycemia and hyperuricemia in college faculty and staff was significantly increased with the increase of BMI. It is suggested that the college employees should have a balanced diet, increase exercise, and control BMI within a reasonable range.
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Objective To investigate the effects of compound cornu cervi degelatinatum on the expression of related inflammatory cytokines and pathological changes of the synovium in mice with collagen-induced arthritis (CIA). MethodsThe CIA model of Balb/c mice was established. The mice were randomly divided into 5 groups: blank control group, model control group, low-dose group ( C1 ) , high-dose group ( C2 ) and glucosidorum tripterygll totorum group D. After the first immunization in mice, we observed the general condition and lesions of the fore and hind jaws. After the second immunization, the mice in groups C1 and C2 were orally administered with compound cornu cervi degelatinatum at the doses of 2. 5 and 5 g·kg-1 body weight per day for 4 weeks, and meanwhile those in group D were administrated with glucosidorum tripterygll totorum at the dose of 13. 6 mg·kg-1 body weight per day. The serum of the mice was collected to detect the levels of TNF-αand IL-4 by ELISA. Ankle joints were harvested, and the pathological changes of synovial tissues were observed under light microscope. Results As compared with the model control group, the level of TNF-αin the treatment groups was significantly decreased, while the level of IL-4 was elevated in group C1. Histological pathology of ankle joints demonstrated that the synovium of the CIA mice were hyperplastic and the synovial tissues were markedly ameliorated in treatment groups. Conclusion The compound cornu cervi degelatinatum can relieve redness and swelling in mice with CIA. The mechanism may be related to the inhibition of pro-inflammatory cytokine TNF-α and regulation of anti-inflammatory cytokine IL-4.
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<p><b>OBJECTIVE</b>To study the correlation among the morphological characteristics and the spica yield of various germplasm of Prunella vulgaris.</p><p><b>METHOD</b>The various P. vulgaris germplasm from all over the country were investigated by analysis of correlation analysis, path analysis and principle component analysis in a randomized block experiment.</p><p><b>RESULT</b>The 6 morphological characteristics were greatly different from each other in various germplasm. The spica yield per plant had a very significantly positive correlation with the number of spica per plant and fresh leaves weight per plant, meanwhile the correlation between the spica yield per plant and spica length was very significantly. Three principal components which accounted for 87.533% of total variance were extracted from the principal component analysis.</p><p><b>CONCLUSION</b>The strong growth potential, the number of spica per plant and spica length were main factors for the selection of high yield breeding of P. vulgaris.</p>
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Genetic Variation , Principal Component Analysis , Prunella , Chemistry , GeneticsABSTRACT
Objective To control the quality of Danlong Kangchi Capsules, fully extract efficiency ingredients and ensure the therapeutic effect by studying the extraction process of the capsule. Methods With the extracting quantity of tanshinoneⅡA, ginsenoside Rb1 and Rg1, Icariin and extract ratio as indexes, the better process parameters with ethanol or water extracting were selected by simple factor test and orthogonal test to Danlong Kangchi Capsules. Results The herbs such as root of red-rooted salvia were extracted by 75% ethanol as a solvent, reflux extracting two times, adding 8 times and 6 times solvent volume respectively, extracting 1.5 and 1.0 hour respectively. Dregs after ethanol extracting and the herbs such as herba Epimedii were decocted by waters soaking 0.5 hour, extracting two times, adding 12 times and 10 times solvent volume respectively, extracting 2.0 and 1.5 hours respectively. Conclusions Extracting processes were validated to be stable and feasible.
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OBJECTIVE:To prepare aspirin gastric floating capsule and to study its in vitro drug-release characteristics.METHODS:Aspirin gastric floating capsule was prepared with aspirin as model drug,and with hydroxypropyl methylcellulose(HPMC)under different viscosity and different quantity and carbopol as main excipients.The release rate of the capsules was determined.RESULTS:When the optimal ratio between HPMC K4M and HPMC K15M was3to1,then the in vitro drug release of aspirin gastric floating capsule within0h～10h conformed to apparent zero-order kinetics with rate constant Kr at10.3%/h and release parameter at0.6173.CONCLUSIONS:The prepared capsule has a marked slow-release effect,the quantity and the viscosity of HPMC were the main factors affecting the release rate of gastric floating capsule,the in vitro drug release characteristics were associated with the joint action of bulk erosion and drug diffusion.
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OBJECTIVE:To establish a method for determination of the contents of chlorogenic acid and baicalin in Shiwuwei Xiaoyanzhike oral liquid .METHODS:HPLC was used,the mobile phase was acetonitrile - acetic acid,the flow rate was 0.8ml/ min and the detecting wavelengths were 326nm and 274nm.RESULTS: The recoveries of chlorogenic acid and baicalin were 96.8% - 104.5% , 94.9% -97.7% and RSD were 1.09% -3.12% , 1.19% -1.76% (n = 3), respectively .CONCLUSION: The method is simple and feasible with a good reproducibility, the RESULTS: can provide a basis for establishing the quality standard of this oral liquid.
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AIM: To establish an effective and convenient method for applying HPLC fingerprints to quality control in the production of Xiasangju Granules(Spica prunellae,Folium mori,Flos chrysanthemi). METHODS: Komasil Sunfrie C_(18)(150 mm?4.6 mm,5 ?m) analytical column was used and eluted with a gradient program consisted of phase A(1% acetic acid) and phase B(methanol) and detected at 290 nm.The fingerprints of aqueous extract,alcohol-precipitated extract,concentrate and finished product were compared with. RESULTS: The fingerprint method for Xiasangju Granules was established.The similarity among 10 batches of Xiasangju Granules was no less than 0.970.The difference between extracts and finished product of Xiasangju Granules was obvious. CONCLUSION: This validated method is available for quality evaluation and quality control in Xiasangju Granule's production.
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AIM:To establish the fingerprint chromatogram of amino acids compounds of Xiasangju Granules(Spica Prunellae,Flos Chrysanthemi indici,Folium mori.).METHODS:To apply 6-aminoqtiinoly-N-hydroxysuccinimdyl carbamate(AQC) pre-column derivatization HPLC method.Separation was performed on SYMMETRY C_(18)(150 mm?(4.6) mm,5 ?m) analytical column with mobile phase consisting of acetate(pH=(5.05)) and 60% acetonitrile with gradient elution with the flow rate(1.0) mL/min and the column temperature at 37 ℃.The Fluorescence wavelength used for detection was set at 250 nm(Excitation wavelength) and 395 nm(Emission wavelength) and the analysis time was 50 min.RESULTS:12 co-peaks on the HPLC fingerprints of Xiasangju Granules were indicated.The similarities were determined by the coefficients of cosine and correlation.The results of similarity analysis were(0.95)-(1.00).CONCLUSION:Perfect fingerprints were obtained which can be used for the quality control of Xiasangju Granules.